As we all know, proteins are essential components when it comes to cells, tissue, and organisms. These molecules are made of long strips of amino acids arranged specifically in 3D format. The side links of the amino acids create lots of space for the chemical interaction to take place. In this, the polypeptides fold themselves into their structures and interact with one another.
Proteins start thousands of biochemical pathways that rules an organism’s function. A study of proteins has revealed information about the function of our body, the paths of disease, and the equation of the genetic code present inside our body. Cytoplasmic and Nuclear protein extraction kit is used for protein extraction.
The main challenge when studying proteins is to choose the appropriate method for protein extraction. What tissue do you want to isolate from protein? Do you identify specifically which protein you want to isolate, or are you looking for a broad range of samples? Once you have specified that, then you can proceed with your protein extraction experiment to match your experimental goal.
The protein extraction process is performed with mechanical or chemical techniques. The first step of protein extraction involves mechanical disruptions such as cutting, smashing, or shearing tissue into smaller pieces. If your target is intracellular proteins, then detergents will need to be used to break the phospholipid cellular membrane.
The Sonication process can also be used to disrupt the membranes of a cell. Most of the time, protease inhibitors are added to the mixture to prevent loss of protein due to enzymatic degradation. So, you can use high-quality nuclear protein extraction kits to perform the protein extraction process.